Distinguishing isomeric N-methylhistidine peptidoforms by ion mobility mass spectrometry

Chem. Commun., 2025, Accepted Manuscript DOI: 10.1039/D5CC03712C, Communication Nurgül Bilgin, Francis Berthias, Tina Ravnsborg, Sadaf Ahmad, Paulina Emmel, Julia Z Kaminska, Jakub Drozak, Ole Jensen, Jasmin Mecinović Histidine residues in proteins undergo histidine methyltransferase-catalysed Nτ-His and Nπ-His methylation. Here, we demonstrate that ion mobility spectrometry enables separation and identification of peptides possessing the isomeric Nτ-methyl-His and Nπ-methyl-His...