Macrocyclization of backbone N-methylated peptides by a prolyl oligopeptidase with a distinctive substrate recognition mechanism

Chem. Sci., 2025, Accepted Manuscript DOI: 10.1039/D5SC03723A, Edge Article Emmanuel Matabaro, Haigang Song, Lukas Sonderegger, Fabio Gherlone, Andrew Giltrap, Samuel Liver, Alvar Gossert, Markus Künzler, James H Naismith Macrocyclization and multiple backbone N-methylations can significantly improve the pharmacological properties of peptides. Since chemical synthesis of such compounds is often challenging, enzyme-based production platforms are an interesting option. Here,...