Thermal proteome profiling of itaconate interactome in macrophages

Chem. Sci., 2025, Advance Article DOI: 10.1039/D5SC02378E, Edge Article Yunzhu Meng, Tiantian Wei, Chenlin Zhang, Anqi Yu, Yuan Liu, Junyu Xiao, Chu Wang Thermal proteome profiling identified interacting proteins of the immunoregulatory metabolite, itaconate, in macrophages. A novel target, mitochondrial branched-chain-amino-acid aminotransferase (BCAT2), was verified to be inhibited by itaconate.