Multi-STEM MePCR: a bisulfite-free, multiplex, high-sensitive and high-specific assay to measure DNA methylation

Chem. Sci., 2025, Accepted Manuscript DOI: 10.1039/D5SC01429H, Edge Article Hao Yang, Jiani Qiu, Yaping Xu, Wei Ren, LinQing Zhen, Gaolian Xu, Hongchen Gu, Hong Xu Aberrant CpG island methylation serves as a pivotal biomarker for cancer diagnostics, with accuracy substantially enhanced by analyzing multiple loci. Current techniques, such as bisulfite conversion or restriction enzyme-based methods,...