Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study

Chem. Commun., 2025, Advance Article DOI: 10.1039/D5CC00971E, Communication Monika Halat, Magdalena Klimek-Chodacka, Agnieszka Domagała, Grzegorz Zając, Tomasz Oleszkiewicz, Josef Kapitán, Rafal Baranski We present a new non-destructive approach to detect CRISPR-Cas biomolecules and distinguish active from inactive forms using Raman optical activity (ROA) and sensitive Eu(III)-probes inducing circularly polarized luminescence (CPL).