Deaminase-Driven Random Mutation Enables Efficient DNA Mutagenesis for Protein Evolution

Chem. Sci., 2025, Accepted Manuscript DOI: 10.1039/D5SC00861A, Edge Article Ying Hao, Tong-Tong Ji, Shu-Yi Gu, Shan Zhang, Yao-Hua Gu, Xia Guo, Li Zeng, Fang-Yin Gang, Jun Xiong, Yu-Qi Feng, Neng-Bin Xie, Bi-Feng Yuan Protein evolution has emerged as a crucial tool for generating proteins with novel characteristics. A key step in protein evolution is the mutagenesis of protein-coding DNA. Error-prone PCR (epPCR) is...