Dual deconvolution in multiphoton structured illumination microscopy for deep-tissue super-resolution imaging

The authors present a computational adaptive optics framework for multiphoton structured illumination microscopy, enabling deep-tissue super-resolution imaging with minimal hardware modifications. Their dual deconvolution algorithm independently corrects excitation and emission aberrations, and they achieve a lateral resolution one-fourth of the emission wavelength at a depth of 180 µm in mouse brain tissue.