Embryo metabolite analysis and implantation potential prediction using chemiluminescent microfluidic chips with dielectric wetting valves

Analysis of embryo metabolites offers a promising non-invasive strategy for assessing preimplantation developmental potential. Here, the authors report a capillary-driven chemiluminescence microfluidic device equipped with three electrowetting valves that enables quantification of glucose, lactate and pyruvate from as little as 3 μL of spent blastocyst culture medium.