Chem. Sci., 2025, Accepted Manuscript DOI: 10.1039/D5SC05343A, Edge Article Keigo Nonaka, Hayato Sakai, Ryusei Mori, Naoki Shimada, Shunsuke Hatatsu, Taku Hasobe, Yoshimitsu Sagara Rotaxane-based mechanophores that exploit spatial separation between a luminophore and a quencher are attractive due to their high structural design flexibility, enabling high-contrast changes in fluorescence intensity. However, it remains...