Subunit fusion unlocks rapid in vitro maturation for slowly activating heterodimeric [FeFe]-hydrogenases

Hydrogenases, particularly the heterodimeric [FeFe]-hydrogenase of Desulfovibrio desulfuricans ATCC 7757 (DdHydAB), present a sustainable alternative to noble metals for catalyzing both hydrogen oxidation and production due to their exceptional catalytic properties. This specific enzyme is highlighted as the most promising variant among hydrogenases for these reactions. The text introduces the research context, which focuses on exploring sustainable catalysis for hydrogen fuel applications. The goal of the work is to leverage the enzymatic efficiency of DdHydAB as a key technology for H₂-related processes.